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Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
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Updated: Jul 5, 2025

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

Future Paths in Cryogenic Single-Molecule Fluorescence Spectroscopy.

Subhasis Adhikari¹, Robert Smit¹, Michel Orrit¹

¹Huygens-Kamerlingh Onnes Laboratory, Leiden University, 2300 RA Leiden, The Netherlands.

The Journal of Physical Chemistry. C, Nanomaterials and Interfaces

|January 17, 2024

Summary

This summary is machine-generated.

Cryogenic single-molecule fluorescence spectroscopy offers detailed insights into molecular interactions and quantum information applications. This technique uses ultranarrow spectral lines to sense local perturbations, paving the way for integrated quantum photonics.

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Area of Science:

Physical Chemistry
Quantum Optics
Spectroscopy

Background:

Cryogenic single-molecule fluorescence spectroscopy has advanced understanding of molecular photophysics and interactions over 30 years.
Zero-phonon lines serve as sensitive probes for molecular dynamics and external perturbations.

Purpose of the Study:

To review advances in cryogenic optical microscopy.
To explore future perspectives in molecular-scale sensing and quantum information applications.

Main Methods:

Utilizing cryogenic single-molecule fluorescence spectroscopy.
Employing ultranarrow lifetime-limited zero-phonon lines as sensors.
Investigating single aromatic hydrocarbon molecules for nanoscale probing.

Main Results:

Demonstrated average-free understanding of molecular photophysics and interactions.
Identified zero-phonon lines as sensitive indicators of local molecular environments.
Highlighted potential of aromatic hydrocarbons in quantum photonics.

Conclusions:

Cryogenic spectroscopy provides unparalleled molecular-scale insights.
Aromatic hydrocarbon molecules are promising for integrated quantum photonics.
Future developments will likely focus on enhanced nanoscale sensing and quantum information technologies.