Leveraging Machine Learning for Size and Shape Analysis of Nanoparticles: A Shortcut to Electron Microscopy

Characterizing nanoparticles (NPs) is crucial in nanoscience due to the direct influence of their physiochemical properties on their behavior. Various experimental techniques exist to analyze the size and shape of NPs, each with advantages, limitations, proneness to uncertainty, and resource requirements. One of them is electron microscopy (EM), often considered the gold standard, which offers visualization of the primary particles. However, despite its advantages, EM can be expensive, less accessible, and difficult to apply during dynamic processes. Therefore, using EM for specific experimental conditions, such as observing dynamic processes or visualizing low-contrast particles, is challenging. This study showcases the potential of machine learning in deriving EM parameters by utilizing cost-effective and dynamic techniques such as dynamic light scattering (DLS) and UV-vis spectroscopy. Our developed model successfully predicts the size and shape parameters of gold NPs based on DLS and UV-vis results. Furthermore, we demonstrate the practicality of our model in situations in which conducting EM measurements presents a challenge: Tracking in situ the synthesis of 100 nm gold NPs.