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Updated: Jul 5, 2025
Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
Published on: October 6, 2022
Split G-quadruplex based PfAgo sensing platform for nucleotide mutation discrimination and human genotyping
Yan Zhang1, Bin Gong1, Yanan Lin1
1School of Pharmacy, Nantong University, Nantong, Jiangsu 226001, China. sugaoxing@ntu.edu.cn.
Abstract:
A PfAgo-G4 sensing platform exploiting G4 as a signal reporter was proposed, validated, and optimized. By introducing two mismatches at the Link strand, a universal nucleotide design rule was established for accurate single nucleotide polymorphism discrimination with PfAgo-G4. The FUT2 gene was then successfully and accurately genotyped using human buccal swab samples.
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