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Updated: Jul 27, 2026

In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage
Published on: January 11, 2012
Endonuclease IV and T4 ligase enhanced detection of mutations in low abundance
1Department of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot, 010020, China. chywang@imu.edu.cn.
Abstract:
We propose a mutant detection approach based on endonuclease IV and DNA ligase in combination with qPCR. The enzymes functioned cooperatively to facilitate PCR for low abundance DNA detection. We demonstrate that our approach can distinguish mutations as low as 0.01%, indicating the potential application of this strategy in early cancer diagnosis.
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