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Mass Spectrum

Mass Spectrum

A mass spectrum is the graphical representation of the relative abundance of the charged fragments in an analyte plotted against their mass-to-charge ratio (m/z). The plot's x axis represents the ratio of the mass of the charged fragment to the elementary charge it carries. The y axis of the plot represents the relative abundance of each charged species. The relative abundance is calculated from the signal intensity of each charged species recorded at the detector. The most intense signal...

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Updated: Jul 5, 2025

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

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Published on: May 29, 2019

Mass Spectral Library for DNA Adductomics.

Scott J Walmsley^1,2, Jingshu Guo^2,3,4, Anamary Tarifa⁵

¹Institute for Health Informatics, University of Minnesota, Minneapolis, Minnesota 55455, United States.

Chemical Research in Toxicology

|January 17, 2024

Summary

This summary is machine-generated.

DNA adducts, formed by environmental factors, can cause cancer if unrepaired. A new spectral database and portal aid in detecting and characterizing these DNA adducts to advance cancer research.

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Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

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DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments

Published on: January 27, 2016

Area of Science:

Environmental Health
Molecular Biology
Toxicology

Background:

DNA adducts form from endogenous electrophiles, radiation, and environmental chemicals.
Unrepaired DNA adducts in critical genes can lead to mutations and cancer.
Accurate detection and quantification of DNA adducts are crucial for understanding carcinogenesis and risk assessment.

Purpose of the Study:

To establish a comprehensive DNA adduct mass spectral database.
To facilitate the detection and characterization of known and novel DNA adducts.
To create a centralized resource for the DNA adductomics research community.

Main Methods:

Collected synthetic DNA adduct standards from researchers.
Acquired mass spectrometry (MS) fragmentation spectra using Orbitrap and Q-TOF instruments.
Processed spectral data and integrated it into the MassBank of North America (MoNA) database.
Developed a DNA adduct portal website for data access and download.

Main Results:

A downloadable spectral library of DNA adducts has been created.
The DNA adduct portal serves as a central repository for mass spectra and metadata.
The database facilitates the identification of previously observed and new DNA adducts.

Conclusions:

The developed spectral library and portal are valuable resources for DNA adductomics research.
This initiative accelerates research into the role of DNA adducts in disease.
Improved understanding of DNA adducts will enhance disease risk assessment and prevention strategies.