[Mechanism of change in the fluorescence parameters of pyrene and diphenylhexatriene in irradiated membranes]

The erythrocyte ghosts were irradiated with doses of 100 to 1000 Gy. The fluorescence intensity and the lifetime of the excited state of pyrene and diphenylhexatriene were shown to decrease. The analysis of the results obtained has demonstrated that the changes in the fluorescence parameters of these probes are related to the enhanced dynamic probe quenching, the quencher being placed in water or the water itself being a quencher.