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CRISPRmap: Sequencing-free optical pooled screens mapping multi-omic phenotypes in cells and tissue
Jiacheng Gu1, Abhishek Iyer1, Ben Wesley1
1Department of Biological Sciences, Columbia University, NY, USA.
Biorxiv : the Preprint Server for Biology
|January 18, 2024
Summary
CRISPRmap is a new optical pooled screening method that analyzes spatial phenotypes and gene function without cell lysis. This multi-omic approach identifies disease-causing mutations and enhances understanding of tissue biology.
Area of Science:
- Genomics and Molecular Biology
- Cell Biology
- Biotechnology
Background:
- Pooled genetic screens are essential for high-throughput gene function studies.
- Traditional sequencing-based screens are limited by cell lysis, hindering the analysis of spatial phenotypes like cell morphology and interactions.
- Optical pooled screening methods offer a solution for investigating spatial phenotypes in response to genetic perturbations.
Conclusions:
- CRISPRmap offers a powerful multi-omic approach for studying spatial phenotypes and gene function in situ.
- The method advances the analysis of DNA damage repair pathways and the functional consequences of genetic variants.
- CRISPRmap has the potential to significantly expand the understanding of tissue biology in health and disease through multi-omic spatial analysis.
