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Pan-cancer Analysis Identifies AIMP2 as a Potential Biomarker for Breast Cancer
Jie Qiu1, Tao Zhou2, Danhong Wang3
1Department of Breast and Thyroid Surgery, Shaoxing People's Hospital, Shaoxing 312000, Zhejiang, China.
Introduction:
Aminoacyl tRNA synthetase complex interacting with multifunctional protein 2 (AIMP2) is a significant regulator of cell proliferation and apoptosis. Despite its abnormal expression in various tumor types, the specific functions and effects of AIMP2 on tumor immune cell infiltration, proliferation, and migration remain unclear.
Materials And Methods:
To assess AIMP2's role in tumor immunity, we conducted a pan-cancer multi-database analysis using the Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), and Cancer Cell Lines Encyclopedia (CCLE) datasets, examining expression levels, prognosis, tumor progression, and immune microenvironment. Additionally, we investigated AIMP2's impact on breast cancer (BRCA) proliferation and migration using cell counting kit 8 (CCK-8) assay, transwell assays, and western blot analysis.
Results:
Our findings revealed that AIMP2 was overexpressed in 24 tumor tissue types compared to normal tissue and was associated with four tumor stages. Survival analysis indicated that AIMP2 expression was strongly correlated with overall survival (OS) in certain cancer patients, with high AIMP2 expression linked to poorer prognosis in five cancer types.
Conclusion:
Finally, siRNA-mediated AIMP2 knockdown inhibited BRCA cell proliferation and migration in vitro. In conclusion, our pan-cancer analysis suggests that AIMP2 may play a crucial role in tumor immunity and could serve as a potential prognostic marker, particularly in BRCA.
Insights
Aminoacyl tRNA synthetase complex interacting with multifunctional protein 2 (AIMP2) is overexpressed in many cancers and linked to poor prognosis. Knocking down AIMP2 inhibited breast cancer cell growth and migration, suggesting AIMP2 as a potential cancer biomarker.
Area of Science:
- Oncology
- Molecular Biology
- Immunology
Background:
- Aminoacyl tRNA synthetase complex interacting with multifunctional protein 2 (AIMP2) regulates cell proliferation and apoptosis.
- AIMP2's role in tumor immunity, including immune cell infiltration, proliferation, and migration, is not fully understood.
Purpose of the Study:
- To investigate the pan-cancer role of AIMP2 in tumor immunity and its potential as a prognostic marker.
- To determine the effect of AIMP2 on breast cancer (BRCA) cell proliferation and migration.
Main Methods:
- Pan-cancer analysis using TCGA, GTEx, and CCLE datasets for AIMP2 expression, prognosis, and immune microenvironment.
- In vitro experiments including CCK-8, transwell assays, and western blot to assess AIMP2's impact on BRCA cells.
Main Results:
- AIMP2 was overexpressed in 24 tumor types and correlated with tumor stage.
- High AIMP2 expression was linked to poorer overall survival in five cancer types.
- AIMP2 knockdown suppressed BRCA cell proliferation and migration.
Conclusions:
- AIMP2 plays a significant role in tumor immunity across various cancers.
- AIMP2 may serve as a valuable prognostic biomarker, especially in breast cancer.
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