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Isolation of CD133+ Liver Stem Cells for Clonal Expansion
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CDC123 promotes Hepatocellular Carcinoma malignant progression by regulating CDKAL1
Yong Wang1, HongTao Pan1, XuanKun Gong2
1The Clinical College, Anhui No.2 Provincial People's Hospital, Anhui Medical University, Hefei 230041, Anhui, China.
Pathology, Research and Practice
|January 18, 2024
Summary
High expression of cell proliferation protein 123 (CDC123) correlates with poor prognosis in hepatocellular carcinoma (HCC). CDC123 knockdown inhibits HCC cell growth, suggesting it
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Cell proliferation protein 123 (CDC123) is implicated in eukaryotic translation.
- CDC123 is a candidate oncogene in breast cancer, but its role in Hepatocellular Carcinoma (HCC) is unknown.
Purpose of the Study:
- To investigate the expression and prognostic significance of CDC123 in HCC.
- To elucidate the role of CDC123 in HCC progression and its potential molecular mechanisms.
Main Methods:
- Utilized TCGA database for CDC123 RNA-seq and clinical data.
- Performed Kaplan-Meier and multivariate Cox analyses for prognostic evaluation.
- Conducted CDC123 knockdown experiments in HCC cell lines.
Main Results:
- CDC123 was significantly upregulated in HCC tissues.
- High CDC123 expression was associated with poorer HCC patient prognosis and identified as an independent prognostic factor.
- CDC123 knockdown suppressed HCC cell proliferation, invasion, and migration.
- CDC123 was co-expressed with CDKAL1, with CDKAL1 levels decreasing upon CDC123 silencing.
Conclusions:
- CDC123 is a potential oncogene promoting HCC progression.
- CDC123 may exert its oncogenic effects by regulating CDKAL1.
- CDC123 represents a promising therapeutic target for Hepatocellular Carcinoma.
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