The response and factors of microbial aerosol emission from the sludge bio-drying process

Exposure to high levels of microbial contaminants during waste disposal leads to the development of various diseases, including respiratory symptoms and gastrointestinal infections. In this study, the emissions of airborne bacteria and fungi during the process of sludge bio-drying were investigated. The recorded emission levels of airborne bacteria and fungi were 2398 ± 1307 CFU/m³ and 1963 ± 468 CFU/m³, respectively. Viable bacteria were sized between 1.1 and 3.3 μm, while fungal particles were concentrated between 2.1 and 4.7 μm. High-throughput sequencing was used to conduct a microbial population assay, and correlation analysis was performed to estimate the relationship between key factors and bioaerosol emissions. The main bacteria identified were Bacillus sp., Lysinibacillus sp. YS11, unclassified Enterobacteriaceae, Brevundimonas olei, and Achromobacter sp.; the primary types of fungi were Aspergillus ochraceus, Gibberella intricans, Fusarium concentricum, Aspergillus qinqixianii, and Alternaria sp.; and the dominant opportunistic pathogens were Bacillus anthracis and Aspergillus ochraceus. At lower moisture and temperature levels, airborne bacterial concentrations were higher, especially the release of fine particles. In addition, moisture content had a significant impact on the microbial population in bioaerosols. This study provides insights into strategies for controlling bioaerosols in the exhaust gases of the sludge bio-drying process.