Related Experiment Video
Updated: Jul 5, 2025

Autofluorescence Imaging to Evaluate Red Algae Physiology
Published on: February 17, 2023
Synergism Variation between intracellular Glutathione, phycocyanin and SOD in microalgae by carbon quantum dot
Shuyu Liu1, Zitong Wu2, Xin Min2
1School of Environment and Chemical Engineering, Shanghai University, Shanghai 201800, PR China; Shanghai Zhixi Technology Co., Ltd, Shanghai 201815, PR China; Shaanxi Key Laboratory of Environmental Monitoring and Forewarning of Trace Pollutants, ShaanXi Xi'an 710054, PR China.
Abstract:
Based on the use of CQDs as fluorescent probe and covalent coupling method to detect biological molecules with amino groups, to deeply analysis and detect the metabolism of Microcystis aeruginosa. The metabolic changes of carboxyl biomolecules in Microcystis aeruginosa were analyzed by covalent coupling method, including GSH, phycocyanin and SOD enzyme. The changes of GSH content and its correlation between phycocyanin, SOD were analyzed. The content of phycocyanin and SOD reached the maximum on the 65th day, and GSH was more sensitive to the growth and metabolism of microalgae. GSH plays an important role in reducing the external oxidative damage of microalgae cells. The synthesis of glutathione (GSH), GSH/GSSG mutual transformation, the production of phytochelating peptide (PC), the ASA-GSH cycle, and other physiological processes are interconnected. These interactions are crucial for preserving the antioxidant properties of microalgae and regulating redox-sensitive signal transduction.

