Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

5.7K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
5.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preserving Shell Integrity of Microgastropods With the Nondestructive HotSHOT DNA Extraction Method.

Ecology and evolution·2026
Same author

Toward scalable species descriptions for dark taxa: the role of molecular data.

ZooKeys·2026
Same author

Morphology-based phylogeny of longhorn beetles (Coleoptera, Cerambycidae) aligns with phylogenomics and informs higher-level systematics.

Cladistics : the international journal of the Willi Hennig Society·2026
Same author

A new species of <i>Lomechusoides</i> (Coleoptera, Staphylinidae) from <i>Formica polyctena</i> anthills in Northeastern Europe.

ZooKeys·2026
Same author

Accuracy of occurrence and abundance estimates from insect metabarcoding.

bioRxiv : the preprint server for biology·2026
Same author

Balancing Barcoding and Genomics: gDNA Quality in Insect Vouchers After HotSHOT DNA Extraction.

Molecular ecology resources·2026

Related Experiment Video

Updated: Jul 5, 2025

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

83.5K

Express barcoding with NextGenPCR and MinION for species-level sorting of ecological samples.

Cristina Vasilita1, Vivian Feng2,3, Aslak Kappel Hansen2

  • 1Stuttgart State Museum of Natural History, Stuttgart, Germany.

Molecular Ecology Resources
|January 19, 2024
PubMed
Summary

A new, cost-effective DNA barcoding workflow enables rapid species identification in field settings. This simplified method is faster and more accurate than traditional sorting, making it ideal for biodiversity discovery and pest identification.

Keywords:
ONT sequencerexpress barcodingnanopore sequencingrapid identificationspecies-level sorting

More Related Videos

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
12:37

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization

Published on: April 14, 2016

38.7K
A Highly Scalable Approach to Perform Ecological Surveys of Selfing Caenorhabditis Nematodes
09:10

A Highly Scalable Approach to Perform Ecological Surveys of Selfing Caenorhabditis Nematodes

Published on: March 1, 2022

2.6K

Related Experiment Videos

Last Updated: Jul 5, 2025

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

83.5K
Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
12:37

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization

Published on: April 14, 2016

38.7K
A Highly Scalable Approach to Perform Ecological Surveys of Selfing Caenorhabditis Nematodes
09:10

A Highly Scalable Approach to Perform Ecological Surveys of Selfing Caenorhabditis Nematodes

Published on: March 1, 2022

2.6K

Area of Science:

  • Molecular Biology
  • Biodiversity Science
  • Bioinformatics

Background:

  • DNA barcoding is crucial for specimen identification and biodiversity discovery.
  • Current methods are often too costly or require specialized labs for time-sensitive applications.
  • Underutilization persists in fields like citizen science, pest identification, and food authentication.

Purpose of the Study:

  • To present a simplified, rapid, and cost-effective DNA barcoding workflow.
  • To demonstrate its utility for time-sensitive species identification and discovery.
  • To enable broader application of DNA barcoding in diverse research and applied settings.

Main Methods:

  • Combined rapid DNA extraction (HotSHOT) with NextGenPCR thermocyclers for amplicon production.
  • Utilized low-cost MinION sequencers for DNA sequencing.
  • Integrated BLAST for specimen identification within the workflow.

Main Results:

  • Successfully generated 250 DNA barcodes for 285 specimens within 6 hours.
  • The workflow requires minimal, benchtop-compatible equipment (Thermocycler, NextGenPCR, Qubit, MinION).
  • Achieved rapid and accurate species-level sorting.

Conclusions:

  • This simplified workflow offers a faster, more accurate, and cost-effective alternative to traditional morpho-species sorting.
  • It democratizes DNA barcoding for applications previously limited by cost and equipment.
  • Facilitates rapid species discovery and identification in diverse, field-based scenarios.