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Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect

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Finback: a web-based data collection system at SSRF biological macromolecular crystallography beamlines.

Feng Yu¹, Ke Liu¹, Huan Zhou¹

¹The Division of Life Science, Shanghai Advanced Research Institute, Chinese Academy of Sciences, 239 Zhengheng Road, Pudong, Shanghai 201204, People's Republic of China.

Journal of Synchrotron Radiation

|January 19, 2024

Summary

A new software system, Finback, enhances macromolecular crystallography (MX) data collection at Shanghai Synchrotron Radiation Facility beamlines. This user-friendly system enables automated, interactive, and remote data collection, improving experimental efficiency.

Keywords:

data collection graphical user interface macromolecular crystallography beamline synchrotron beamline control

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Area of Science:

Structural Biology
Biophysics
Materials Science

Background:

Macromolecular crystallography (MX) is crucial for determining 3D structures of biological molecules.
Automated data collection at synchrotron beamlines enhances experimental throughput and efficiency.
Current systems may lack user-friendly interfaces or robust remote access capabilities.

Purpose of the Study:

To describe the development and features of Finback, an integrated software system for MX data collection.
To provide a user-friendly, web-based graphical user interface (GUI) for interactive and automated data collection.
To enable simultaneous on-site and remote access to synchrotron beamlines.

Main Methods:

Development of a web-based GUI using modern web technologies (WebSocket, WebGL, WebWorker, WebAssembly).
Integration of Finback with BL02U1 and BL10U2 beamlines at Shanghai Synchrotron Radiation Facility.
Implementation of features for automated MX data collection and support for multiple concurrent sessions.

Main Results:

Finback provides a user-friendly web-based GUI for interactive MX data collection.
The system supports simultaneous on-site and remote access, enhancing beamline accessibility.
Finback automatically deposits experimental parameters and results into a database, integrating with data management systems.

Conclusions:

Finback offers an advanced, integrated software solution for automated macromolecular crystallography data collection.
The system's web-based GUI and concurrent session support improve user experience and beamline utilization.
Finback facilitates efficient and accessible MX experiments at synchrotron facilities.