Unveiling angular sweep total fluorescence Spectroscopy:A novel multidimensional technique for analysis of complex...

Area of Science:

• Analytical Chemistry
• Spectroscopy

Background:

• Analyzing complex multi-fluorophoric systems often requires extensive sample pre-processing.
• Unconventional fluorescence techniques and chemometrics are typically employed to overcome these challenges.

Purpose of the Study:

• Introduce Angular Sweep Total Fluorescence Spectroscopy (ASTFS) as a novel multidimensional steady-state fluorescence technique.
• Demonstrate ASTFS's efficacy in analyzing analytes within complex matrices with minimal pre-processing.

Main Methods:

• ASTFS involves acquiring variable angle synchronous fluorescence spectra across a specific spectral region.
• The technique strategically avoids Rayleigh scattering signals and blind regions, minimizing data matrix size.
• Instrumental configurations and spectral acquisition parameters for ASTFS were investigated.

Main Results:

• ASTFS exhibits enhanced spectral resolution due to a band-narrowing effect.
• The technique proved effective for analyzing samples with strong background fluorescence, exemplified by milk.
• Norfloxacin was successfully quantified in milk samples with minimal pre-processing, achieving excellent analytical figures of merit.

Conclusions:

• ASTFS is a powerful new tool for analyzing complex fluorescent systems.
• The method simplifies analysis by reducing or eliminating the need for sample pre-processing.
• ASTFS offers a promising approach for the quantification of analytes in challenging real-world samples.