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Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells
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MinD-RNase E interplay controls localization of polar mRNAs in E. coli
Shanmugapriya Kannaiah1,2, Omer Goldberger3, Nawsad Alam3,4
1Department of Microbiology and Molecular Genetics, IMRIC, The Hebrew University Faculty of Medicine, P.O.Box 12272, 91120, Jerusalem, Israel. kannaiah@wustl.edu.
The EMBO Journal
|January 19, 2024
Summary
The MinD protein and RNase E enzyme control which messenger RNAs (mRNAs) are found at bacterial cell poles. Their interaction shapes the polar transcriptome, impacting protein localization and cell function.
Area of Science:
- Microbiology
- Molecular Biology
- Cell Biology
Background:
- The bacterial cell pole transcriptome is unique, but mechanisms for mRNA polar localization are unknown.
- Factors mediating mRNA localization to membranes are suggested, but polar-specific mechanisms remain elusive.
Purpose of the Study:
- To identify factors regulating messenger RNA (mRNA) localization to bacterial cell poles.
- To elucidate the molecular mechanisms governing polar mRNA distribution in E. coli.
Main Methods:
- Candidate system approach combined with proteomics.
- In silico modeling and experimental validation.
- Analysis of RNase E dynamics and mRNA stability in mutant strains.
Main Results:
- MinD protein identified as essential for polar mRNA localization, interacting with RNase E.
- RNase E's membrane-binding site mediates MinD interaction, influencing RNase E dynamics.
- Polar accumulation of RNase E in ΔminCDE cells led to mRNA destabilization and depletion from poles.
Conclusions:
- The interplay between MinD and RNase E dictates the polar transcriptome composition.
- MinD and RNase E play crucial roles in mRNA polar localization and subsequent protein product localization.
- This study assigns novel functions to MinD and RNase E in bacterial cell polarity and gene expression regulation.
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