Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Editing02:23

RNA Editing

9.0K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.0K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

9.3K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
9.3K
Leaky Scanning02:28

Leaky Scanning

5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

10.6K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
10.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hijacking the host heparan sulfate-cell-surface ribonucleoproteins (RNPs) scaffold pathway for viral immune evasion.

Virologica Sinica·2026
Same author

Bergenin Suppresses Glycolysis and Malignant Progression in Breast Cancer via the IGF1R-MAPK Signaling Pathway.

Annals of clinical and laboratory science·2026
Same author

[Preparation and identification of mouse monoclonal antibody against human adenovirus type 55 Fiber protein].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology·2026
Same author

Current status on encephalitic alphavirus vaccines development: Advances, challenges, and global health perspectives.

Infectious medicine·2026
Same author

[Recombinant expression of rift valley fever virus nucleocapsid protein and generation of rabbit polyclonal antibodies].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology·2026
Same author

Parvimonas micra-derived SirTM: An ADP-ribosyltransferase as a novel driver in colorectal cancer progression.

Journal of advanced research·2026
Same journal

ADAM17 blockade restores natural killer cell antibody-dependent cytotoxicity against human immunodeficiency virus-1.

Virology journal·2026
Same journal

A receptor-Fc based SFV replicon Trim-Away platform for targeted viral protein degradation.

Virology journal·2026
Same journal

Molecular characterization and phylogenetic analysis of foot-and-mouth disease virus in Nigeria.

Virology journal·2026
Same journal

Genomic insights into influenza A(H1N1)pdm09 virus evolution in Yunnan province, China, during 2018-2023.

Virology journal·2026
Same journal

Serotypes distribution characteristic of enterovirus in sewage in Hangzhou, Zhejiang Province from January 2023 to December 2024.

Virology journal·2026
Same journal

Viral metagenomic analysis of the blood virome in patients with multiple autoimmune diseases.

Virology journal·2026
See all related articles

Related Experiment Video

Updated: Jul 5, 2025

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
12:20

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

21.4K

N6-methyladenosine modification positively regulate Japanese encephalitis virus replication.

Min Yao1, Zhirong Cheng1,2, Xueyun Li1,3

  • 1Department of Microbiology, Airforce Medical University, Xi'an, 710032, Shaanxi, China.

Virology Journal
|January 19, 2024
PubMed
Summary
This summary is machine-generated.

N6-methyladenosine (m6A) modification positively regulates Japanese encephalitis virus (JEV) replication. METTL3 knockdown significantly reduces JEV replication and enhances the host immune response, revealing m6A

Keywords:
FlaviviridaJapanese encephalitis virusMETTL3MeRIP-seqN6-Methyladenosine modification

More Related Videos

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

10.9K
Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
14:40

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

3.3K

Related Experiment Videos

Last Updated: Jul 5, 2025

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
12:20

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

21.4K
A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

10.9K
Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
14:40

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

3.3K

Area of Science:

  • Virology
  • Molecular Biology
  • Epigenetics

Background:

  • N6-methyladenosine (m6A) modification is found in viral RNA, regulating virus replication and host immunity.
  • The specific role of m6A in Japanese encephalitis virus (JEV) replication remained uninvestigated.

Purpose of the Study:

  • To investigate the role of m6A modification in regulating JEV replication in mouse neuroblast cells (neuro2a).

Main Methods:

  • Detection of m6A modification in the JEV genome during infection.
  • Analysis of METTL3 (an m6A writer) expression in infected mouse brain tissue.
  • siRNA-mediated knockdown of METTL3 to assess its impact on JEV replication and host innate immunity.

Main Results:

  • The JEV genome exhibits m6A modification in neuro2a cells.
  • JEV infection led to decreased METTL3 expression in mouse brain.
  • METTL3 knockdown significantly reduced JEV replication and progeny virus production at 48 hours post-infection (hpi).
  • METTL3 knockdown cells showed an increased innate immune response to JEV infection.

Conclusions:

  • m6A modification plays a positive role in JEV infection.
  • Distinct m6A signatures exist for both JEV and the host during infection.
  • This study enhances the understanding of m6A's role in Flaviviridae virus infections.