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Updated: Jul 5, 2025
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Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
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Long- and short-read sequencing methods discover distinct circular RNA pools in Lotus japonicus
Asa Budnick1, Megan J Franklin1, Delecia Utley1
1Department of Plant and Microbial Biology, North Carolina State University, Raleigh, North Carolina, USA.
The Plant Genome
|January 20, 2024
Summary
Researchers identified nearly 6000 novel circular RNAs (circRNAs) in Lotus japonicus, overcoming challenges in circRNA discovery. This study provides a valuable resource for understanding circRNA functions in plants.
Area of Science:
- Molecular Biology
- Genomics
- Plant Science
Background:
- Circular RNAs (circRNAs) are RNA molecules formed by a unique back-splicing mechanism.
- Their low abundance and sequence similarity to linear RNAs pose challenges for identification.
- Understanding circRNAs is crucial for gene regulation studies in plants.
Purpose of the Study:
- To identify and characterize novel circRNAs in Lotus japonicus.
- To compare different methodologies for circRNA discovery and validation.
- To assess the conservation of circRNA-expressing genes across plant species.
Main Methods:
- Enrichment, amplification, and sequencing of RNA from Lotus japonicus leaf tissue.
- Application of diverse bioinformatics pipelines for circRNA identification.
- Validation using reverse transcription polymerase chain reaction and nanopore sequencing.
Main Results:
- Identification of nearly 6000 novel circRNAs in Lotus japonicus.
- Methodologies yielded distinct circRNA pools with limited overlap.
- Validated circRNAs showed conservation in gene expression across plant species.
Conclusions:
- This study presents the first comprehensive identification of circRNAs in Lotus japonicus.
- The findings offer a foundational resource for investigating circRNA functions in gene regulation.
- Comparative analysis of methods provides insights for future circRNA research in plants.
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