Mitochondrial protein CHCHD10 inhibits NDV replication and reduces pathological changes.

Area of Science:

• Veterinary Virology
• Molecular Biology
• Immunology

Background:

• Newcastle disease (ND) poses a significant threat to the global poultry industry.
• The pathogenic mechanisms of virulent Newcastle disease virus (NDV) are not fully understood.
• Proteomic analysis of NDV-infected cells is crucial for understanding host-pathogen interactions.

Purpose of the Study:

• To investigate the role of coiled-coil-helix-coiled-coil-helix domain containing 10 (CHCHD10) protein during NDV infection.
• To determine the expression patterns of CHCHD10 in response to NDV.
• To assess the impact of CHCHD10 expression levels on viral load and disease severity.

Main Methods:

• Proteomic analysis of NDV-infected cells.
• In vivo and in vitro detection of CHCHD10 expression after NDV infection.
• Assessment of viral loads and pathological damage in C57BL/6 mice with altered CHCHD10 expression.

Main Results:

• CHCHD10 protein levels significantly decreased at the late stage of NDV infection.
• CHCHD10 expression was notably reduced both in vivo and in vitro following NDV infection.
• Silencing CHCHD10 in mice led to increased viral loads and exacerbated pathological damage, while overexpression had the opposite effect.

Conclusions:

• CHCHD10 plays a protective role against Newcastle disease virus infection.
• Reduced CHCHD10 expression is associated with increased NDV pathogenicity.
• CHCHD10 may represent a potential therapeutic target for managing Newcastle disease.