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FBXW7 polymorphism asserts susceptibility to colorectal cancer
Laraib Uroog1, Bushra Zeya1, Khalid Imtiyaz1
1Genome Biology Lab, Department of Biosciences, Jamia Millia Islamia, New Delhi 110025, India.
Gene
|January 20, 2024
Summary
Single nucleotide polymorphisms (SNPs) in the FBXW7 gene are linked to colorectal cancer (CRC) risk. The rs6842544 SNP is associated with CRC development, potentially serving as a biomarker for high-risk individuals.
Area of Science:
- Genetics
- Oncology
- Molecular Biology
Background:
- FBXW7, an F-Box protein family member, is a candidate cancer susceptibility gene.
- Single nucleotide polymorphisms (SNPs) in FBXW7 may influence cancer risk.
- Colorectal cancer (CRC) pathogenesis warrants further investigation into genetic factors.
Purpose of the Study:
- To investigate the association between FBXW7 gene polymorphisms and colorectal cancer risk.
- To analyze the functional impact of FBXW7 SNPs on CRC development.
- To evaluate FBXW7 expression in CRC tissues.
Main Methods:
- Case-control study with 450 CRC patients and 450 healthy controls.
- Genotyping of FBXW7 SNPs (rs2255137 and rs6842544) using PCR-RFLP and SSCP, confirmed by sequencing.
- In-silico analysis, western blotting, and RT-PCR for functional and expression studies.
Main Results:
- A significant association was found between the FBXW7 rs6842544 SNP and colorectal cancer risk.
- The homozygous CC genotype for rs6842544 showed a slightly increased risk (OR=1.590).
- The CC haplotype was linked to good prognosis, while the TT haplotype reduced CRC risk. FBXW7 expression was lower in CRC tissues.
Conclusions:
- The FBXW7 rs6842544 polymorphism is associated with colorectal cancer risk.
- FBXW7 rs6842544 may serve as a molecular biomarker for screening high-risk CRC populations.
- Genetic variations in FBXW7 play a role in colorectal cancer pathogenesis.
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