Global regulator IrrE on stress tolerance: a review

Area of Science:

• Microbiology
• Molecular Biology
• Biotechnology

Background:

• Stress tolerance is crucial for survival against environmental challenges.
• Deinococcus radiodurans possesses remarkable radiation resistance.
• IrrE (PprI) is a global regulator involved in DNA damage response (DDR) in D. radiodurans.

Purpose of the Study:

• To review the regulatory role and mechanism of IrrE in D. radiodurans's radiation response.
• To summarize the applications and effects of heterologous IrrE expression in enhancing abiotic stress tolerance.

Main Methods:

• Review of existing literature on IrrE function and regulation.
• Analysis of molecular and systems-level impacts of heterologously expressed IrrE.
• Investigation of gene, protein, module, and pathway regulation by IrrE.

Main Results:

• IrrE enhances resilience to radiation, heat, oxidation, osmotic stress, and inhibitors in bacteria, fungi, plants, and mammalian cells.
• Heterologous expression of IrrE has been engineered to broaden its stress tolerance applications.
• IrrE mediates the transcription of DDR genes, acting as a global regulator.

Conclusions:

• IrrE is a key factor in D. radiodurans's radiation resistance.
• IrrE holds significant potential for engineering stress tolerance in various organisms.
• Understanding IrrE's regulatory mechanisms can advance biotechnology for stress resilience.