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Integrated Point-of-Care Immune Cell Analyzer with Rapid Blood Sample Reaction and Wide Field-of-View Detection.

Jakir Hossain Imran1, Mohiuddin Khan Shourav2, Jung Kyung Kim3

  • 1Department of Mechanical Engineering, Graduate School, Kookmin University, Seoul 02707, Republic of Korea.

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|January 22, 2024
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Summary

We developed a low-cost, automated cell analyzer for rapid immune response monitoring. This device uses a rotating cartridge and fluorescence imaging for accurate blood analysis at the point-of-care.

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Area of Science:

  • Biomedical Engineering
  • Immunology
  • Analytical Chemistry

Background:

  • Conventional automated diagnostic devices often rely on expensive robotic fluid handling.
  • There is a need for affordable, reliable, and rapid diagnostic tools for monitoring immune responses from blood samples.

Purpose of the Study:

  • To develop an integrated cell analyzer for automated, cost-effective immune cell analysis.
  • To improve the efficiency and accuracy of antigen-antibody reactions and cell counting.

Main Methods:

  • An integrated cell analyzer was developed with a cylindrical sample cartridge, a DC motor, and a fluorescence imaging module.
  • Automated sample mixing and loading were controlled via an Android application and motor rotation.
  • A curved detection chamber and a single-lens fluorescence imaging module were used for aberration-free imaging.

Main Results:

  • Active mixing in the cartridge reduced antigen-antibody reaction time by 3.3-fold.
  • Cell counting accuracy improved, and reagent consumption decreased 4-fold compared to conventional methods.
  • The curved detection chamber enabled wide-field-of-view, aberration-free fluorescence imaging.

Conclusions:

  • The developed integrated cell analyzer provides a practical and cost-effective solution for point-of-care immune response monitoring.
  • The device automates sample handling and analysis, enhancing diagnostic capabilities.