Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Manganese(II) Oxidation and Mineralization by the Deep-Sea Bacterium Shewanella piezotolerans WP3.

Geobiology·2026
Same author

Single-Cell Reveal GALNT7-Dependent Ferroptosis Suppression as a Mechanism of Immunotherapy Resistance in Non-Small Cell Lung Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Multimodal Nanobubbles Carrying Indocyanine Green and VCAM-1 Targeting Peptide for Molecular Imaging of DOX-Induced Cardiotoxicity.

International journal of nanomedicine·2026
Same author

Targeting immunoglobulin superfamily member 9 (IGSF9) to overcome acute myeloid leukemia resistance to CAR-T therapy.

Journal of experimental & clinical cancer research : CR·2026
Same author

Removing Pb<sup>2+</sup> ions from aqueous solution with freshwater cyanobacteria: Combined adsorption and intracellular precipitation.

Journal of hazardous materials·2026
Same author

Saikosaponin D Alleviates Liver Ischemia-Reperfusion Injury by Inhibiting Mitophagy-Associated Ferroptosis via the STAT3/PINK1 Pathway.

Phytotherapy research : PTR·2026

Related Experiment Video

Updated: Jul 5, 2025

Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-&#947;-Dependent Cell Autonomous Immunity
11:21

Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity

Published on: March 12, 2015

11.1K

Protozoan-Derived Cytokine-Transgenic Macrophages Reverse Hepatic Fibrosis.

Ying Chen1,2,3, Jie Wang1,2, Nan Zhou1,2

  • 1Department of Biochemistry and Molecular Biology, Research Center for Infectious Diseases, School of Basic Medical Sciences, Anhui Medical University, Hefei, 230032, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|January 22, 2024
PubMed
Summary
This summary is machine-generated.

Engineered macrophages expressing a Toxoplasma gondii cytokine offer a safer, more effective therapy for liver fibrosis by improving immune cell recruitment and function. This novel approach enhances macrophage-based immunotherapy for treating liver fibrosis.

Keywords:
Toxoplasma gondiiToxoplasma gondii macrophage migration inhibitory factorsimmunotherapyliver fibrosismacrophagespolarization

More Related Videos

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation
07:46

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Published on: April 3, 2017

25.3K
Depletion and Reconstitution of Macrophages in Mice
08:50

Depletion and Reconstitution of Macrophages in Mice

Published on: August 1, 2012

39.7K

Related Experiment Videos

Last Updated: Jul 5, 2025

Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-&#947;-Dependent Cell Autonomous Immunity
11:21

Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity

Published on: March 12, 2015

11.1K
Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation
07:46

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Published on: April 3, 2017

25.3K
Depletion and Reconstitution of Macrophages in Mice
08:50

Depletion and Reconstitution of Macrophages in Mice

Published on: August 1, 2012

39.7K

Area of Science:

  • Immunology
  • Cell Biology
  • Parasitology

Background:

  • Macrophage therapy shows promise for liver fibrosis but requires safer, more defined cell phenotypes.
  • Macrophage heterogeneity poses challenges for consistent therapeutic outcomes in liver fibrosis.

Purpose of the Study:

  • To develop a novel macrophage-based immunotherapy for liver fibrosis.
  • To engineer macrophages with a stable phenotype expressing a key cytokine from Toxoplasma gondii.

Main Methods:

  • Generated transgenic macrophages (Mφtgmif) expressing Toxoplasma gondii macrophage migration inhibitory factor (TgMIF).
  • Assessed Mφtgmif fibrinolytic and chemotactic capabilities.
  • Evaluated Mφtgmif efficacy in ameliorating liver fibrosis in vivo.
  • Investigated the mechanisms of Mφtgmif action, including immune cell recruitment and polarization.

Main Results:

  • Mφtgmif demonstrated stable fibrinolysis and enhanced chemotactic capacity.
  • Mφtgmif effectively reduced liver fibrosis by recruiting and polarizing endogenous macrophages via CCL2 and CX3CL1.
  • TgMIF activation of the ERK/HMGB1/NF-κB pathway promoted CCL2 expression, enhancing macrophage recruitment.
  • Mφtgmif showed superior therapeutic effects, higher chemotaxis, and lower inflammation compared to LPS/IFN-γ-treated macrophages.

Conclusions:

  • Engineered Mφtgmif provides a safer and more efficient macrophage-based immunotherapy for liver fibrosis.
  • TgMIF acts as an evolutionarily designed immunomodulator to modify the liver's immune microenvironment.
  • This strategy offers a viable approach for treating liver fibrosis and potentially other human diseases.