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Updated: Jul 5, 2025

Induction and Validation of Cellular Senescence in Primary Human Cells
08:18

Induction and Validation of Cellular Senescence in Primary Human Cells

Published on: June 20, 2018

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Vutiglabridin Alleviates Cellular Senescence with Metabolic Regulation and Circadian Clock in Human Dermal

Jin-Woong Heo1,2, Hye-Eun Lee3,4, Jimin Lee1

  • 1School of Undergraduate Studies, Daegu Gyeongbuk Institute of Science and Technology, College of Transdisciplinary Studies, Daegu 42988, Republic of Korea.

Antioxidants (Basel, Switzerland)
|January 22, 2024
PubMed

Insights

Vutiglabridin alleviates cellular senescence and restores circadian rhythm by improving mitochondrial function. This anti-obesity compound shows promise for treating aging-associated diseases by targeting the link between senescence, metabolism, and the circadian clock.

Area of Science:

  • Gerontology
  • Cell Biology
  • Metabolomics

Background:

  • Cellular senescence, marked by cell cycle arrest, is linked to metabolic dysfunction and disrupted circadian rhythms.
  • Obesity exacerbates aging by increasing oxidative stress and mitochondrial burden, impacting these interconnected processes.
  • Vutiglabridin, an anti-obesity molecule, enhances mitochondrial function.

Purpose of the Study:

  • To investigate the effects of vutiglabridin on cellular senescence, metabolism, and circadian rhythm.
  • To determine if vutiglabridin can alleviate senescence markers and restore circadian clock function.
  • To explore the therapeutic potential of vutiglabridin in aging-associated diseases.

Main Methods:

  • Chronic treatment of senescent primary human dermal fibroblasts (HDFs) with vutiglabridin.
  • Assessment of senescence markers (SA-β-gal, CDKN1A, CDKN2A).
  • Evaluation of circadian rhythm markers (BMAL1) and mitochondrial function/structure.

Main Results:

  • Vutiglabridin treatment reduced key markers of cellular senescence.
  • The compound restored the dysfunctional cellular circadian rhythm (BMAL1).
  • Vutiglabridin prevented mitochondrial alterations associated with senescence and improved metabolic regulation.

Conclusions:

  • Vutiglabridin demonstrates significant senescence-alleviating effects.
  • The drug restores cellular circadian rhythmicity and metabolic regulation.
  • These findings support vutiglabridin's potential for aging-associated diseases, highlighting the senescence-metabolism-circadian clock link.
Keywords:
cellular senescencecircadian clockshuman dermal fibroblastsmetabolismmitochondrial homeostasis

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