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Related Experiment Video

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Comparison of Retinal Metabolic Activity and Structural Development between rd10 Mice and Normal Mice Using

Erin Su1, Niranjana Kesavamoorthy1, Jason A Junge2

  • 1Department of Ophthalmology, USC Roski Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA.

Current Issues in Molecular Biology
|January 22, 2024
PubMed
Summary

This study used fluorescence lifetime imaging microscopy (FLIM) to reveal accelerated metabolic changes in rd10 mouse retinas compared to controls. These findings offer insights into retinal degeneration and potential therapeutic strategies.

Keywords:
FLIMNAD(P)Hglycolysismultiphoton fluorescence lifetime imaging microscopyoxidative phosphorylationretinaretinal degenerationretinal development

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Area of Science:

  • Biomedical Optics
  • Retinal Physiology
  • Metabolic Imaging

Background:

  • Metabolic state is crucial for tissue function and disease progression.
  • Nicotinamide adenine dinucleotide (NAD(P)H) fluorescence lifetimes reflect cellular metabolic activity.
  • Retinal degeneration models provide insights into disease mechanisms.

Purpose of the Study:

  • To investigate metabolic changes during retinal development in C57BL6/J (wild-type) and rd10 (retinal degeneration model) mice.
  • To compare the metabolic profiles of developing retinas using multiphoton fluorescence lifetime imaging microscopy (FLIM).
  • To understand the role of oxidative phosphorylation and glycolysis in retinal development and degeneration.

Main Methods:

  • Multiphoton fluorescence lifetime imaging microscopy (FLIM) was employed.
  • Analysis focused on the fluorescence lifetimes of free and bound nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate (NAD(P)H).
  • Developing retinas from C57BL6/J and rd10 mice were analyzed at various time points up to 3 months post-birth.

Main Results:

  • Oxidative phosphorylation initially decreased then increased in both mouse strains, plateauing over time.
  • This metabolic shift occurred earlier in rd10 mice (postnatal day 10) compared to C57BL6/J mice (postnatal day 30).
  • Higher oxidative phosphorylation rates were observed in the inner retina compared to the outer retina in both strains.

Conclusions:

  • The rd10 mouse model exhibits accelerated metabolic maturation of the retina.
  • FLIM analysis of NAD(P)H provides a valuable tool for studying retinal metabolic changes.
  • Understanding these metabolic dynamics can inform strategies for treating retinal degeneration.