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Sequencing Reveals miRNAs Enriched in the Developing Mouse Enteric Nervous System.
Christopher Pai1,2, Rajarshi Sengupta3, Robert O Heuckeroth1,2
1The Children's Hospital of Philadelphia Research Institute, Philadelphia, PA 19104, USA.
Non-Coding RNA
|January 22, 2024
Summary
Researchers identified key microRNAs (miRNAs) in the developing enteric nervous system (ENS). However, they found that miR-137 loss did not impact ENS development or survival in mice, challenging previous findings.
Area of Science:
- Developmental biology
- Neuroscience
- Genetics
Background:
- The enteric nervous system (ENS) is crucial for gut function.
- Hirschsprung disease (HSCR) arises from ENS developmental defects.
- MicroRNA (miRNA) alterations are linked to HSCR, but the normal developing ENS miRNA profile is unclear.
Purpose of the Study:
- To characterize the miRNA landscape in the developing ENS.
- To investigate the role of specific miRNAs, including miR-137, in ENS development.
Main Methods:
- Small RNA sequencing (smRNA-seq) and mRNA sequencing (mRNA-seq) were performed on mouse ENS precursor cells.
- RNA was compared between ENS precursor cells and other developing bowel cells.
- Genetic manipulation was used to assess the function of miR-137 in vivo.
Main Results:
- 73 miRNAs were significantly enriched in the developing ENS.
- Top 5 conserved miRNAs include miR-9, miR-27b, miR-124, miR-137, and miR-488.
- Loss of miR-137 in various neural crest lineages did not affect mouse survival or ENS development.
Conclusions:
- This study provides a comprehensive miRNA profile of the developing ENS.
- The findings challenge the previously reported role of miR-137 in ENS development.
- Further research is needed to understand ENS development and HSCR pathogenesis.
