[Nucleolus-forming regions in the chromosomes in continuous cell cultures]

Localization of nucleoli-forming (NF) areas in chromosomes of continuous cell cultures of African green monkey kidney Vero, CV-1, GMK, BGM, BS-C-1. Macaca rhesus kidney LLC-MK2, dog kidney MDCK, pig kidney SPEV, PK-15 was studied. The NF-areas in chromosomes of African green monkey, M. rhesus, and pig cell cultures were shown to be localized in sites of secondary chromosomal stangulation, and there was also a definite correlation between the intensity of staining of NF-areas and sizes of the zone of secondary strangulation. In chromosomes of MDCK cell line, NF-areas are located in telomere ends of six-nine chromosomes, and association of two chromosomes by NF-areas was observed. The possibility of using the method of identification of nucleoi-forming areas for the evaluation of productivity of continuous cell cultures is discussed.