Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>IN VITRO</i> EFFICACY OF DISINFECTANTS AGAINST <i>NANNIZZIOPSIS ARTHROSPORIOIDES</i>.

Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians·2026
Same author

Detection of Viable <i>Nannizziopsis guarroi</i> in Housing Environments Prior to Dermatological Lesion Development in Bearded Dragons (<i>Pogona vitticeps</i>).

Animals : an open access journal from MDPI·2026
Same author

Update on Lower Urinary Disorders of Exotic Companion Mammals.

The veterinary clinics of North America. Exotic animal practice·2025
Same author

<i>Nannizziopsis arthrosporioides</i> infection mimicking ophidiomycosis in ball pythons (<i>Python regius</i>).

Medical mycology case reports·2025
Same author

Amphibians With Dermatological Disease: A Retrospective Study of 223 Cases at Five University Veterinary Teaching Hospitals (1986-2024).

Veterinary dermatology·2025
Same author

THE EFFECTS OF ANTICOAGULANT AND TIME ON HEMATOLOGIC VALUES IN RED-TAILED HAWKS (<i>BUTEO JAMAICENSIS</i>).

Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians·2025

Related Experiment Video

Updated: Jul 5, 2025

Administration of Volatile Anesthetics to Zebrafish Larvae for Behavioral Observation
04:55

Administration of Volatile Anesthetics to Zebrafish Larvae for Behavioral Observation

Published on: June 6, 2025

187

Anesthesia and humane euthanasia methods for king worm larvae (Zophobas morio).

Amber M Lum1, Krista A Keller1,2

  • 1Department of Veterinary Clinical Medicine, University of Illinois College of Veterinary Medicine, Urbana, Illinois, USA.

Zoo Biology
|January 22, 2024
PubMed
Summary

Humane euthanasia methods for king worms (Zophobas morio) were evaluated. Ethanol submersion and potassium chloride injection at 3 mg/g achieved 100% mortality, suitable for food sources.

Keywords:
insectisofluranemortality ratewelfare

More Related Videos

Double Direct Injection of Blood into the Cisterna Magna as a Model of Subarachnoid Hemorrhage
10:34

Double Direct Injection of Blood into the Cisterna Magna as a Model of Subarachnoid Hemorrhage

Published on: August 30, 2020

10.5K
In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal
05:25

In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal

Published on: February 10, 2021

4.8K

Related Experiment Videos

Last Updated: Jul 5, 2025

Administration of Volatile Anesthetics to Zebrafish Larvae for Behavioral Observation
04:55

Administration of Volatile Anesthetics to Zebrafish Larvae for Behavioral Observation

Published on: June 6, 2025

187
Double Direct Injection of Blood into the Cisterna Magna as a Model of Subarachnoid Hemorrhage
10:34

Double Direct Injection of Blood into the Cisterna Magna as a Model of Subarachnoid Hemorrhage

Published on: August 30, 2020

10.5K
In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal
05:25

In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal

Published on: February 10, 2021

4.8K

Area of Science:

  • Veterinary Science
  • Entomology
  • Animal Welfare

Background:

  • Growing concerns regarding insect welfare necessitate humane euthanasia protocols.
  • King worms (Zophobas morio) are commercially important darkling beetle larvae.
  • Current euthanasia methods for insects lack comprehensive evaluation.

Purpose of the Study:

  • To assess prolonged isoflurane exposure for king worm euthanasia.
  • To evaluate secondary euthanasia methods for king worms.
  • To identify humane and effective euthanasia for edible insect larvae.

Main Methods:

  • Larvae exposed to isoflurane for varying durations (10 min to 24 h).
  • Recovery times monitored post-isoflurane exposure.
  • Secondary methods tested: potassium chloride injection, ethanol, formalin, water immersion, and freezing.

Main Results:

  • Prolonged isoflurane exposure extended recovery but did not ensure 100% mortality.
  • Ethanol submersion and 3 mg/g potassium chloride injection achieved 100% mortality.
  • Freezing and formalin immersion were ineffective euthanasia methods.

Conclusions:

  • Isoflurane is insufficient for king worm euthanasia.
  • Ethanol and high-dose potassium chloride are effective euthanasia methods for king worms.
  • Humane euthanasia methods are crucial for edible insect production.