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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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Depth sectioning using environmental and atomic-resolution STEM.

Masaki Takeguchi1, Ayako Hashimoto1, Kazutaka Mitsuishi1

  • 1Center for Basic Research on Materials, National Institute for Materials Science, 1-2-1 Sengen, Tsukuba, Ibaraki 305-0047, Japan.

Microscopy (Oxford, England)
|January 22, 2024
PubMed
Summary
This summary is machine-generated.

Depth-sectioning scanning transmission electron microscopy (STEM) improves image resolution for samples in gas or liquid environments. This technique enhances signal-to-background ratio, enabling atomic-level imaging with environmental cells.

Keywords:
3DDepth sectioningSTEMatomic resolutionenvironmentalgasliquid

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Area of Science:

  • Materials Science
  • Microscopy
  • Nanotechnology

Background:

  • Environmental cell (EC) transmission electron microscopy (TEM) enables imaging of samples in gas and liquid media using silicon nitride membranes.
  • Achieving high resolution in EC-TEM is challenged by the signal-to-background ratio.
  • Depth-sectioning scanning TEM (STEM) offers a method to enhance signal for samples within a matrix.

Purpose of the Study:

  • To introduce depth-sectioning STEM as a technique for high-resolution imaging.
  • To review the applications of depth-sectioning STEM in environmental cells.
  • To highlight the potential for atomic-level resolution in gas and liquid environments.

Main Methods:

  • Utilizing depth-sectioning scanning TEM (STEM) principles.
  • Employing environmental cells (EC) with silicon nitride membranes for sample containment.
  • Acquiring scanning transmission electron microscopy (TEM) images in various media.

Main Results:

  • Depth-sectioning STEM enhances the signal-to-background ratio for samples.
  • The technique allows for increased resolution, potentially to the atomic level.
  • Successful application in imaging samples within gas and liquid media.

Conclusions:

  • Depth-sectioning STEM is a powerful technique for high-resolution environmental transmission electron microscopy.
  • It overcomes resolution limitations posed by signal-to-background ratios in EC-TEM.
  • Enables advanced applications in studying materials and processes in their native gaseous or liquid states.