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The Secreted Ly6/uPAR-Related Protein 1 (Slurp1) Modulates Corneal Angiogenic Inflammation Via NF-κB Signaling.

Sudha Swamynathan1, Gregory Campbell1, Peri Sohnen1

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Secreted Ly-6/uPAR related protein 1 (SLURP1) normally prevents excessive blood vessel growth in the cornea. Its absence in Slurp1-null mice leads to increased inflammation and blood vessel formation after injury.

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Area of Science:

  • Ophthalmology
  • Immunology
  • Molecular Biology

Background:

  • Secreted Ly-6/uPAR related protein 1 (SLURP1) is present in corneal epithelium and tears.
  • SLURP1 has demonstrated antiangiogenic properties.

Purpose of the Study:

  • To investigate the role of SLURP1 in corneal neovascularization and inflammation.
  • To characterize the corneal response to silver nitrate (AgNO3) cautery in Slurp1-null (Slurp1X-/-) mice compared to wild-type (WT) mice.

Main Methods:

  • Corneal neovascularization (CNV) and immune cell infiltration were assessed using CD31 and CD45 staining.
  • Macrophage and neutrophil infiltration were quantified by flow cytometry.
  • Gene expression analysis (VEGFA, MMP2, IL-1b, vimentin) and NF-κB pathway component evaluation were performed.

Main Results:

  • Slurp1X-/- corneas showed significantly denser CNV and increased immune cell infiltration post-AgNO3 cautery compared to WT.
  • Upregulation of VEGFA, MMP2, IL-1b, and vimentin transcripts was observed in Slurp1X-/- corneas.
  • NF-κB signaling was found to be constitutively active in naive Slurp1X-/- corneal epithelium.

Conclusions:

  • SLURP1 deficiency exacerbates corneal angiogenic inflammation.
  • SLURP1 appears to modulate corneal angiogenic inflammation through the NF-κB signaling pathway.