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In Vitro Fertilization

In Vitro Fertilization

In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...

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Related Experiment Video

Updated: Jul 5, 2025

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model

Published on: May 16, 2019

Efficient and repeatable in vitro fertilization in rabbits.

J G Hamze¹, P Peris-Frau², B Galiano-Cogolludo²

¹Animal Reproduction Department, INIA, CSIC, Spain; Department of Cell Biology and Histology, Universidad de Murcia, International Excellence Campus for Higher Education and Research (Campus Mare Nostrum), Murcia, Spain.

|January 22, 2024

Summary

This summary is machine-generated.

Developing a consistent in vitro fertilization (IVF) technique for rabbits involved optimizing sperm preparation and media. Brackett and Oliphant (BO) medium significantly improved sperm parameters and fertilization rates, leading to high embryo development.

Keywords:

Acrosome reaction Gamete In vitro fertilization Oocyte Rabbit Sperm

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Area of Science:

Reproductive Biology
Animal Science
In Vitro Fertilization

Background:

Rabbit in vitro fertilization (IVF) presents significant challenges for gamete interaction studies and artificial reproductive techniques.
Optimizing sperm parameters and fertilization media is crucial for successful rabbit IVF.

Purpose of the Study:

To develop a consistent and efficient in vitro fertilization (IVF) protocol for rabbits.
To evaluate the effects of different media and sperm preparation methods on rabbit sperm parameters and fertilization outcomes.

Main Methods:

Ejaculated rabbit sperm were purified using density gradients and incubated in Tyrode's Albumin Lactate Pyruvate (TALP), human tubal fluid (HTF), or Brackett and Oliphant (BO) media.
Cumulus-oocyte complexes (COCs) were collected at different time points post-ovulation induction and co-incubated with sperm.
Sperm parameters (viability, motility, capacitation) and fertilization rates (pronuclear formation) were assessed.

Main Results:

Brackett and Oliphant (BO) medium significantly enhanced total and progressive sperm motility and linearity compared to TALP and HTF.
Pronuclear formation rates were significantly higher (∼84%) in BO medium, irrespective of sperm pre-incubation.
COCs collected 12 hours post-ovulation induction in BO medium yielded high cleavage (91%) and blastocyst (59%) rates.

Conclusions:

Brackett and Oliphant (BO) medium is superior for rabbit sperm preparation and in vitro fertilization (IVF).
A consistent rabbit IVF protocol involves density gradient-purified sperm co-incubated with COCs collected 12 hours post-ovulation induction in BO medium.
This optimized protocol supports efficient fertilization and subsequent embryo development in vitro.