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Updated: Jun 26, 2026

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Bioluminescent Bacterial Imaging In Vivo
Published on: November 4, 2012
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An improved pathway for autonomous bioluminescence imaging in eukaryotes
Ekaterina S Shakhova1,2, Tatiana A Karataeva1,2, Nadezhda M Markina1,2
1Planta LLC, Moscow, Russia.
Nature Methods
|January 22, 2024
Abstract:
The discovery of the bioluminescence pathway in the fungus Neonothopanus nambi enabled engineering of eukaryotes with self-sustained luminescence. However, the brightness of luminescence in heterologous hosts was limited by performance of the native fungal enzymes. Here we report optimized versions of the pathway that enhance bioluminescence by one to two orders of magnitude in plant, fungal and mammalian hosts, and enable longitudinal video-rate imaging.
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