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Updated: Jul 5, 2025
A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
Published on: November 14, 2018
Novel 3D-Printed Microfluidic Magnetic Platform for Rapid DNA Isolation
Güneş Kibar1,2,3, Büşra Sarıarslan3,4, Serkan Doğanay5
1Department of Materials Science and Engineering, Adana Alparslan Türkeş Science and Technology University, Adana 01250, Turkey.
Abstract:
This study presents a novel miniaturized device as a 3D-printed microfluidic magnetic platform specifically designed to manipulate magnetic microparticles in a microfluidic chip for rapid deoxyribonucleic acid (DNA) isolation. The novel design enables the movement of the magnetic particles in the same or opposite directions with the flow or suspends them in continuous flow. A computational model was developed to assess the effectiveness of the magnetic manipulation of the particles. Superparamagnetic monodisperse silica particles synthesized in-house are utilized for the isolation of fish sperm DNA and human placenta DNA. It was demonstrated that the proposed platform can perform DNA isolation within 10 min with an isolation efficiency of 50% at optimum operating conditions.
