Genome-Wide Identification and Expression Analysis of the SBP-Box Gene Family in Loquat Fruit Development
Haiyan Song1,2,3, Ke Zhao1,2, Guoliang Jiang1,2
1Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China.
Genes
|January 23, 2024
Summary
Researchers identified 28 SBP-box genes in loquat, finding two key genes, EjSBP01 and EjSBP19, potentially regulating carotenoid synthesis and fruit ripening in loquat fruit development.
Area of Science:
- Plant genetics and molecular biology
- Fruit development and ripening
- Nutrient biosynthesis in plants
Background:
- Loquat (Eriobotrya japonica) fruit is valued for its medicinal properties and nutrients like carotenoids.
- SBP-box genes are crucial for plant growth, but their role in loquat fruit development is unknown.
- Understanding loquat fruit ripening and carotenoid accumulation requires identifying relevant genes.
Purpose of the Study:
- To identify and characterize the SBP-box gene family in the loquat genome.
- To investigate the expression patterns of loquat SBP-box genes during fruit development.
- To identify candidate SBP-box genes involved in carotenoid biosynthesis and fruit ripening in loquat.
Main Methods:
- Genome-wide identification of 28 EjSBP genes in loquat.
- Phylogenetic analysis, gene structure, conserved motif, and cis-element analysis of EjSBP proteins.
- Quantitative real-time PCR (qRT-PCR) to analyze gene expression in different tissues and fruit types (white- vs. yellow-fleshed).
Main Results:
- Twenty-eight EjSBP genes were identified and characterized based on genomic distribution, phylogeny, and structure.
- Most EjSBP genes were highly expressed in vegetative tissues, with five showing high fruit expression.
- Eight EjSBP genes were differentially expressed between white- and yellow-fleshed fruits, with EjSBP01 and EjSBP19 showing distinct patterns related to carotenoid accumulation.
Conclusions:
- The SBP-box gene family in loquat has been comprehensively identified and characterized.
- EjSBP genes play significant roles in loquat fruit development, particularly at the breaker stage.
- EjSBP01 and EjSBP19 are strong candidates involved in regulating carotenoid synthesis and accumulation during loquat fruit ripening.
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