Adult Animal Stem Cell-Derived Organoids in Biomedical Research and the One Health Paradigm

Area of Science:

• Biomedical Research
• Translational Science
• Organoid Technology

Background:

• Preclinical research faces limitations with traditional in vivo and in vitro models.
• 2D cell cultures fail to replicate complex organ epithelial structures, hindering scientific progress.
• The One Health Initiative promotes integrated human, animal, and environmental health research.

Purpose of the Study:

• To highlight the potential of organoid technology in preclinical biomedical research.
• To explore the application of organoids in translational and reverse translational research.
• To discuss how novel organoid models expand research possibilities.

Main Methods:

• Development of organoid technology to model organ epithelial microstructure and function.
• Derivation of organoids from human, murine, veterinary, and exotic animal species.
• Application of organoid models in comparative biomedical research.

Main Results:

• Organoids offer a more reliable in vitro system compared to traditional 2D cell lines.
• A growing number of organoid models from diverse species are becoming available.
• These models facilitate research in previously underrepresented animal species.

Conclusions:

• Organoid technology significantly enhances the predictiveness of in vitro models.
• Expanded organoid availability opens new avenues for One Health and reverse translational research.
• Organoids from unique animal models offer insights into overcoming medical challenges in both human and veterinary medicine.