CRISPR/Cas9 as a Mutagenic Factor
Andrey R Shumega1, Youri I Pavlov2,3, Angelina V Chirinskaite4
1Department of Genetics and Biotechnology, St. Petersburg State University, 199034 St. Petersburg, Russia.
International Journal of Molecular Sciences
|January 23, 2024
Summary
CRISPR/Cas9 gene editing is powerful but can cause harmful mutations. Researchers reviewed its mutagenic properties and ways to reduce risks for safer genome editing applications.
Area of Science:
- Genetics
- Molecular Biology
- Biotechnology
Background:
- CRISPR/Cas9 gene editing has transformed genetic research and therapeutic applications.
- CRISPR/Cas9 systems induce DNA breaks at target and off-target sites.
- Unintended mutations from DNA repair pathways can lead to diseases.
Purpose of the Study:
- To describe CRISPR/Cas9 as a mutagenic factor.
- To discuss the mutagenic properties of CRISPR/Cas9.
- To review factors influencing CRISPR/Cas9 mutagenicity.
Main Methods:
- Review of CRISPR/Cas9 mutagenic properties.
- Analysis of DNA repair pathways in genome editing.
- Examination of strategies to mitigate off-target effects.
Main Results:
- CRISPR/Cas9 acts as a potent mutagenic agent.
- Off-target mutations and imprecise DNA repair contribute to unwanted genetic alterations.
- Modified Cas9 nucleases, improved delivery, and targeted repair pathways can reduce risks.
Conclusions:
- CRISPR/Cas9 technology requires careful management due to its mutagenic potential.
- Understanding and controlling CRISPR/Cas9-induced mutations are crucial for safe genome editing.
- Future research should focus on enhancing precision and minimizing off-target effects in gene editing applications.
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