Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

51.1K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
51.1K
Homologous Recombination02:31

Homologous Recombination

50.6K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.6K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cathepsin Z/X: Breaking Down the Known and Unknown.

International journal of molecular sciences·2026
Same author

Comparative Analysis of Crystal Violet-Binding Aptamers as Potential Cores for Binary Sensors.

International journal of molecular sciences·2025
Same author

Vulnerable Nucleotide Pools and Genomic Instability in Yeast Strains with Deletion of the <i>ADE12</i> Gene Encoding for Adenylosuccinate Synthetase.

International journal of molecular sciences·2025
Same author

Prion-like Properties of Short Isoforms of Human Chromatin Modifier PHC3.

International journal of molecular sciences·2025
Same author

Unveiling the Roles of Cysteine Proteinases F and W: From Structure to Pathological Implications and Therapeutic Targets.

Cells·2024
Same author

G-Quadruplex Forming DNA Sequence Context Is Enriched around Points of Somatic Mutations in a Subset of Multiple Myeloma Patients.

International journal of molecular sciences·2024
Same journal

Correction: Mahmud et al. Thymoquinone Attenuates NF-κβ Signalling Activation in Retinal Pigment Epithelium Cells Under AMD-Mimicking Conditions. <i>Int. J. Mol. Sci.</i> 2025, <i>26</i>, 11473.

International journal of molecular sciences·2026
Same journal

Correction: Borovikov et al. The Twisting and Untwisting of Actin and Tropomyosin Filaments Are Involved in the Molecular Mechanisms of Muscle Contraction, and Their Disruption Can Result in Muscle Disorders. <i>Int. J. Mol. Sci</i>. 2025, <i>26</i>, 6705.

International journal of molecular sciences·2026
Same journal

Correction: Molagoda et al. Flavonoid Glycosides from <i>Ziziphus jujuba</i> var. <i>inermis</i> (Bunge) Rehder Seeds Inhibit α-Melanocyte-Stimulating Hormone-Mediated Melanogenesis. <i>Int. J. Mol. Sci.</i> 2021, <i>22</i>, 7701.

International journal of molecular sciences·2026
Same journal

Correction: Guo et al. Integrated Transcriptomic and Metabolomic Analysis Reveals the Molecular Regulatory Mechanism of Flavonoid Biosynthesis in Maize Roots Under Lead Stress. <i>Int. J. Mol. Sci.</i> 2024, <i>25</i>, 6050.

International journal of molecular sciences·2026
Same journal

Correction: Chang et al. Improvement of Carbon Tetrachloride-Induced Acute Hepatic Failure by Transplantation of Induced Pluripotent Stem Cells Without Reprogramming Factor c-Myc. <i>Int. J. Mol. Sci.</i> 2012, <i>13</i>, 3598-3617.

International journal of molecular sciences·2026
Same journal

Correction: Pînzariu et al. Gut Microbiota and Short-Chain Fatty Acids: Key Factors in Pediatric Obesity and Therapeutic Targets. <i>Int. J. Mol. Sci.</i> 2025, <i>26</i>, 11503.

International journal of molecular sciences·2026
See all related articles

Related Experiment Video

Updated: Jul 5, 2025

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
10:07

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Published on: August 25, 2017

7.8K

CRISPR/Cas9 as a Mutagenic Factor.

Andrey R Shumega1, Youri I Pavlov2,3, Angelina V Chirinskaite4

  • 1Department of Genetics and Biotechnology, St. Petersburg State University, 199034 St. Petersburg, Russia.

International Journal of Molecular Sciences
|January 23, 2024
PubMed
Summary
This summary is machine-generated.

CRISPR/Cas9 gene editing is powerful but can cause harmful mutations. Researchers reviewed its mutagenic properties and ways to reduce risks for safer genome editing applications.

Keywords:
CRISPR/Cas9genome editingmutationsoff-target activity

More Related Videos

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
10:07

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

Published on: March 16, 2022

2.1K
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

12.6K

Related Experiment Videos

Last Updated: Jul 5, 2025

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
10:07

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Published on: August 25, 2017

7.8K
Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
10:07

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

Published on: March 16, 2022

2.1K
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

12.6K

Area of Science:

  • Genetics
  • Molecular Biology
  • Biotechnology

Background:

  • CRISPR/Cas9 gene editing has transformed genetic research and therapeutic applications.
  • CRISPR/Cas9 systems induce DNA breaks at target and off-target sites.
  • Unintended mutations from DNA repair pathways can lead to diseases.

Purpose of the Study:

  • To describe CRISPR/Cas9 as a mutagenic factor.
  • To discuss the mutagenic properties of CRISPR/Cas9.
  • To review factors influencing CRISPR/Cas9 mutagenicity.

Main Methods:

  • Review of CRISPR/Cas9 mutagenic properties.
  • Analysis of DNA repair pathways in genome editing.
  • Examination of strategies to mitigate off-target effects.

Main Results:

  • CRISPR/Cas9 acts as a potent mutagenic agent.
  • Off-target mutations and imprecise DNA repair contribute to unwanted genetic alterations.
  • Modified Cas9 nucleases, improved delivery, and targeted repair pathways can reduce risks.

Conclusions:

  • CRISPR/Cas9 technology requires careful management due to its mutagenic potential.
  • Understanding and controlling CRISPR/Cas9-induced mutations are crucial for safe genome editing.
  • Future research should focus on enhancing precision and minimizing off-target effects in gene editing applications.