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Association between Resistant Arterial Hypertension, Type 2 Diabetes, and Selected microRNAs
Robert Błaszczyk1, Alicja Petniak2, Jacek Bogucki3
1Department of Cardiology, Medical University of Lublin, 20-090 Lublin, Poland.
Journal of Clinical Medicine
|January 23, 2024
Summary
This study found higher MIR1-1 and MIR195 microRNA levels in patients with Rheumatic Heart Disease (RHD) and Type 2 Diabetes Mellitus (T2DM). These microRNAs may serve as potential biomarkers for RHD and T2DM co-occurrence.
Area of Science:
- Biochemistry
- Genetics
- Cardiology
Background:
- Cardiovascular diseases are closely linked to microRNA (miRNA) expression changes.
- Rheumatic Heart Disease (RHD) and Type 2 Diabetes Mellitus (T2DM) are associated with altered miRNA profiles.
Purpose of the Study:
- To investigate the clinical characteristics of patients with idiopathic RHD.
- To evaluate the expression levels of specific microRNAs in patients with RHD and T2DM.
Main Methods:
- Quantitative real-time polymerase chain reaction (qPCR) was used to determine miRNA levels.
- Gene expression was calculated using the 2-ΔΔCT method.
- 115 RHD patients were analyzed, including 53 with T2DM.
Main Results:
- MIR1-1 and MIR195 gene expression showed statistically significant differences (p < 0.05) in patients with T2DM.
- MIR1-1 expression was significantly higher in RHD patients with T2DM compared to those without T2DM.
- MIR195 expression was also significantly higher in RHD patients with T2DM.
Conclusions:
- Elevated MIR1-1 and MIR195 gene expression levels were observed in patients with co-existing RHD and T2DM.
- Further research is needed to elucidate the precise roles of these miRNAs.
- These microRNAs show potential as clinical biomarkers for RHD and T2DM.
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