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Urodele egg jelly and fertilization.

P Jego, H Lerivray, A Chesnel

Advances in Experimental Medicine and Biology

|January 1, 1986

Summary

Urodele amphibians exhibit polyspermic fertilization due to a lack of early sperm blocking mechanisms. A late block to sperm entry, involving oviductal secretions like lectins, occurs in the egg jelly layers.

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Area of Science:

Reproductive biology
Amphibian fertilization
Biochemistry

Background:

Urodele amphibians exhibit polyspermic fertilization, lacking typical egg surface blocks like cortical reactions.
A late block to sperm entry occurs approximately 30 minutes post-fertilization within the egg jelly layers.
Egg jelly, crucial for sperm capacitation, is secreted by oviductal cells.

Purpose of the Study:

To investigate the role of oviductal secretions in urodele amphibian fertilization.
To characterize the biochemical properties of oviductal secretions and their relation to the late block of sperm entry.

Main Methods:

Biochemical analysis of oviductal secretions from eight urodele species.
Double diffusion experiments on agarose plates to detect component interactions.
Purification and characterization of lectins from oviductal secretions.
Spatial localization of lectins within the egg jelly.

Main Results:

Interactions between anterior and middle oviductal secretions formed precipitin lines, potentially creating the dense zone of the capsular chamber.
Hemagglutinating activity was detected in oviductal secretions, varying by species.
Lectins (18K and 26K) were purified from Ambystoma mexicanum and Pleurodeles waltl oviducts, respectively.
The location of these lectins within the jelly correlated with the site of the late block to sperm entry.

Conclusions:

Oviductal secretions play a significant role in regulating polyspermic fertilization in urodele amphibians.
Specific lectins within the egg jelly appear to be involved in the late block to sperm entry.
While lectins are spatially linked to the fertilization block, they do not induce visible morphological changes in sperm upon direct contact.

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