Related Experiment Video
Updated: Jul 5, 2025
09:12
Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods
Published on: May 11, 2018
6.9K
Phage Lytic Protein CHAPSH3b Encapsulated in Niosomes and Gelatine Films
Verdiana Marchianò1,2, Ana Catarina Duarte3,4, Seila Agún3,4
1Department of Physical and Analytical Chemistry, University of Oviedo, Julián Clavería 8, 33006 Oviedo, Spain.
Microorganisms
|January 23, 2024
Summary
Researchers encapsulated antimicrobial endolysins in niosomes, enhancing their stability and efficacy. These protected endolysins show promise for food industry applications against bacteria like Staphylococcus aureus.
Area of Science:
- Biotechnology
- Food Science
- Microbiology
Background:
- Antimicrobial resistance (AMR) necessitates novel therapeutic strategies beyond conventional antibiotics.
- Bacteriophage-derived endolysins offer potent antimicrobial activity but face stability challenges.
- Encapsulation in delivery systems can improve endolysin stability and controlled release.
Purpose of the Study:
- To encapsulate the chimeric endolysin CHAPSH3b into niosomes for enhanced stability.
- To characterize the CHAPSH3b-loaded niosomes.
- To evaluate the potential application of endolysin-loaded niosomes in the food industry by incorporating them into gelatin films and assessing antimicrobial efficacy against Staphylococcus aureus.
Main Methods:
- Chimeric endolysin CHAPSH3b was encapsulated in non-ionic surfactant-based vesicles (niosomes) using the thin-film hydration (TFH) method.
- Niosomes were characterized for size, zeta potential, and encapsulation efficiency (EE).
- Endolysin-loaded niosomes were incorporated into gelatin films for stability and antimicrobial efficacy testing against Staphylococcus aureus.
Main Results:
- Niosomes containing CHAPSH3b exhibited sizes in the range of 30-80 nm and zeta potentials between 30-50 mV.
- Encapsulation efficiency (EE) for CHAPSH3b within niosomes ranged from 50-60%.
- The study demonstrated the potential for using these endolysin-loaded niosomes in gelatin films for antimicrobial applications.
Conclusions:
- Niosomal encapsulation effectively protects endolysins, improving their stability and potential for therapeutic applications.
- The developed endolysin-loaded niosomes show promise for integration into food products to combat bacterial contamination.
- Further research can explore optimizing niosome formulations and their application in diverse food matrices.
Related Concept Videos
01:30Lytic Cycle of Bacteriophages
70.7K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
70.7K
00:43Lysogenic Cycle of Bacteriophages
62.2K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
62.2K
00:56Viral Structure
62.3K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
62.3K