Development and In Vivo Evaluation of a Novel Vitamin D3 Oral Spray Delivery System

Area of Science:

• Oncology
• Nanotechnology
• Drug Delivery

Background:

• Developing selective and low-toxicity cancer therapeutics is challenging.
• Tumor cells express vitamin D3 receptors, responding to vitamin D3 with growth arrest and cell death.
• Conventional vitamin D3 formulations lack dose control and targeted delivery.

Purpose of the Study:

• To prepare a vitamin D3 nanospray for inhalation delivery.
• To evaluate the physical properties and biological stability of the nanospray formulation.
• To assess the suitability of the nanospray for inhalation routes.

Main Methods:

• Formulation of vitamin D3 nanospray for inhalation.
• Evaluation of particle size distribution and biological stability.
• Assessment of spray characteristics including volume, variance, fan angle, and beam width.

Main Results:

• The optimized vitamin D3 spray demonstrated ease of use, minimal dripping, and rapid drying.
• The formulation maintained biological stability for 3 months under specified storage conditions.
• The nanospray exhibited superior spray characteristics (larger fan angle, wider beam width, symmetry) compared to traditional nasal sprays.

Conclusions:

• Vitamin D3 nanospray is a promising formulation for inhalation delivery in cancer treatment.
• The developed nanospray overcomes limitations of conventional vitamin D3 delivery methods.
• The formulation's enhanced physical and spray properties make it suitable for targeted drug delivery via inhalation.