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Upstream and Downstream Bioprocessing in Enzyme Technology
Nadia Guajardo1, Rodrigo A Schrebler2
1Departamento de Ingeniería Química y Bioprocesos, Escuela de Ingeniería, Pontificia Universidad Católica de Chile, Avenida Vicuña Mackenna 4860, Macul, Santiago 7820436, Chile.
Pharmaceutics
|January 23, 2024
Summary
Integrating upstream and downstream bioprocessing is crucial for efficient enzymatic biocatalysis. Optimizing these stages ensures a sustainable and cost-effective biotransformation system.
Area of Science:
- Biotechnology
- Biochemical Engineering
- Enzymatic Biocatalysis
Background:
- Biotransformation efficiency relies on integrating upstream and downstream processes.
- Downstream processing often represents a significant portion of overall bioprocessing costs.
- Upstream choices directly impact downstream processability and economics.
Purpose of the Study:
- To provide a comprehensive overview of critical upstream and downstream bioprocessing aspects in enzymatic biocatalysis.
- To highlight the interdependence of upstream and downstream stages for successful biotransformation.
- To guide the selection of methodologies for sustainable and productive biocatalytic systems.
Main Methods:
- Review of enzyme production and immobilization techniques.
- Analysis of solvent selection and statistical optimization in upstream processing.
- Examination of biocatalyst recovery and product separation/purification in downstream processing.
Main Results:
- Upstream processes like enzyme production and immobilization significantly influence downstream operations.
- Effective solvent selection and optimization strategies enhance bioprocess performance.
- Efficient biocatalyst recovery and product purification are key to economic viability.
Conclusions:
- The synergistic integration of optimized upstream and downstream processes is essential for developing high-yield, sustainable biotransformation systems.
- Careful consideration of upstream parameters is vital for minimizing downstream costs and maximizing overall productivity.
- This review offers a framework for selecting appropriate methodologies to achieve efficient enzymatic biocatalysis.
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