Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Disease Outcomes in Boys with <i>ABCD1</i> Variants Identified by Newborn Screening for X-ALD.

medRxiv : the preprint server for health sciences·2026
Same author

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026
Same author

Normative modeling for quantitative brain MRI phenotyping and biomarker discovery for pediatric leukodystrophies.

medRxiv : the preprint server for health sciences·2026
Same author

Automated Flushing System for Post-Processing in Microfluidic Device Fabrication.

Micromachines·2026
Same author

Provider Perceptions of the Impact of Rapid Whole Genome Sequencing on Care and Management.

Research square·2026
Same author

BLOC1S1 variants cause lysosomal and autophagic defects resulting in a hypomyelinating leukodystrophy with epileptic encephalopathy.

American journal of human genetics·2026

Related Experiment Video

Updated: Jul 5, 2025

Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon
06:02

Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon

Published on: September 13, 2019

9.8K

Using Electroporation to Improve and Accelerate Zebrafish Embryo Toxicity Testing.

Nusrat Tazin1, Tamara J Stevenson2, Joshua L Bonkowsky2

  • 1Department of Electrical and Computer Engineering, University of Utah, Salt Lake City, UT 84112, USA.

Micromachines
|January 23, 2024
PubMed
Summary

Electroporation enables faster zebrafish embryo toxicity testing by delivering toxins inside the chorion. This method improves toxin exposure, reducing testing time from days to hours for environmental toxicology studies.

Keywords:
electroporationmicroinjectionnanoparticlestoxicity testingzebrafish embryo

More Related Videos

Rapid Evaluation of Toxicity of Chemical Compounds Using Zebrafish Embryos
00:07

Rapid Evaluation of Toxicity of Chemical Compounds Using Zebrafish Embryos

Published on: August 25, 2019

10.7K
In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina
06:55

In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina

Published on: December 27, 2011

19.2K

Related Experiment Videos

Last Updated: Jul 5, 2025

Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon
06:02

Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon

Published on: September 13, 2019

9.8K
Rapid Evaluation of Toxicity of Chemical Compounds Using Zebrafish Embryos
00:07

Rapid Evaluation of Toxicity of Chemical Compounds Using Zebrafish Embryos

Published on: August 25, 2019

10.7K
In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina
06:55

In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina

Published on: December 27, 2011

19.2K

Area of Science:

  • Biomedical research
  • Environmental toxicology
  • Zebrafish models

Background:

  • Zebrafish embryos are valuable for toxicity testing.
  • The chorion limits toxin exposure, potentially causing false negatives.
  • Current fish embryo toxicity testing (FET) is time-consuming.

Purpose of the Study:

  • To develop electroporation for enhanced toxin delivery in zebrafish embryos.
  • To reduce the time required for zebrafish embryo toxicity testing.
  • To improve the sensitivity of FET.

Main Methods:

  • Developed a custom electroporation device for zebrafish embryos (<3 hpf).
  • Optimized electrical parameters (pulse width, number, amplitude) for toxin delivery.
  • Validated the system using Trypan blue dye, gold nanoparticles (AuNPs), and silver nanoparticles (AgNPs).

Main Results:

  • Electroporation successfully delivered dyes and nanoparticles into zebrafish embryos through the chorion.
  • Delivery concentration was controllable via electrical parameters.
  • Electroporation with AgNPs reduced toxicity testing time from 4 days to hours.

Conclusions:

  • Electroporation is an effective method for rapid toxin delivery in zebrafish embryos.
  • This technique significantly shortens FET duration.
  • Electroporation enhances zebrafish embryo toxicity testing and drug delivery applications.