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Hematologic parameters and leukocyte histogram patterns in infectious mononucleosis.
American Journal of Clinical Pathology
|April 1, 1987
Summary
Automated blood analysis using the Coulter S-Plus IV can help identify infectious mononucleosis (IM). The instrument flagged most IM samples, despite normal hemoglobin and platelet counts, highlighting reactive lymphocytes.
Area of Science:
- Hematology
- Clinical Pathology
- Immunology
Background:
- Infectious mononucleosis (IM) is a common viral illness.
- Accurate and efficient laboratory diagnosis is crucial for patient management.
- Automated hematology analyzers play a key role in routine blood testing.
Purpose of the Study:
- To evaluate the performance of the Coulter S-Plus IV automated analyzer in identifying infectious mononucleosis.
- To assess the utility of specific flags and histogram data for IM detection.
Main Methods:
- Analysis of blood samples from 60 patients with confirmed infectious mononucleosis.
- Utilizing the Coulter S-Plus IV for automated leukocyte differentials and histograms.
- Comparison of automated results with manual microscopic differentials.
Main Results:
- Leukocyte counts were elevated in less than half of IM cases.
- Reactive lymphocytes were observed in all patients.
- The Coulter S-Plus IV showed discrepancies in leukocyte differentials compared to manual counts.
- Specific flags (R2, R, H) and histogram abnormalities (wider, asymmetrical lymphocyte peaks) were noted.
- The analyzer correctly identified 59 out of 60 IM samples requiring further review.
Conclusions:
- The Coulter S-Plus IV can assist in identifying infectious mononucleosis.
- Automated flags and histogram analysis provide valuable data for detecting IM.
- Further investigation of flagged samples is essential for accurate diagnosis.