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Related Experiment Video

Updated: Jul 5, 2025

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
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Inflammatory Cytokines Can Induce Synthesis Of Type-I Interferon.

Nikhil Sharma, Xu Qi, Patricia Kessler

    Biorxiv : the Preprint Server for Biology
    |January 23, 2024
    PubMed
    Summary
    This summary is machine-generated.

    Inflammatory cytokines like TNF-α and IL-1β can induce Type I interferon (IFN) production in uninfected cells. This occurs via p38 MAPK activation of PACT, which then activates the RIG-I signaling pathway.

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    Related Experiment Videos

    Last Updated: Jul 5, 2025

    High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
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    A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3
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    Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

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    Area of Science:

    • Immunology
    • Cellular Biology
    • Molecular Signaling

    Background:

    • Type I interferons (IFNs) are crucial for antiviral responses and immune cell development.
    • While IFN induction by viral components is well-understood, the mechanisms in uninfected cells are unclear.
    • Inflammatory cytokines play a role in various diseases and immune processes.

    Approach:

    • Investigated the induction of IFN-β in uninfected cells using inflammatory cytokines TNF-α and IL-1β.
    • Focused on the role of the cytoplasmic RIG-I signaling pathway.
    • Utilized cell lines and primary cells expressing RIG-I and PACT.

    Key Points:

    • Cytokine treatment (TNF-α / IL-1β) activates IFN-β expression.
    • IFN-β induction is mediated by the RIG-I signaling pathway.
    • PACT protein activator of PKR interacts with RIG-I to activate IFN-β expression.

    Conclusions:

    • Discovered a novel pathway for Type I IFN induction by inflammatory cytokines in uninfected cells.
    • p38 MAPK activation leads to PACT phosphorylation, enabling RIG-I signaling.
    • This mechanism reveals how ubiquitous inflammatory signals can trigger IFN responses.