Charting Single Cell Lineage Dynamics and Mutation Networks via Homing CRISPR
Lin Wang1, Wenjuan Dong1, Zheng Yin1,2
1Department of System Medicine and Bioengineering, Houston Methodist Neal Cancer Center, Houston, Texas 77030.
Biorxiv : the Preprint Server for Biology
|January 23, 2024
Summary
LINEMAP precisely traces mutations for single-cell lineage reconstruction using CRISPR-Cas9 and homing guide RNA (hgRNA). This computational framework predicts mutation pathways, advancing disease evolution research and targeted therapy development.
Area of Science:
- Genomics
- Computational Biology
- Molecular Biology
Background:
- Single-cell lineage tracing is crucial for understanding disease evolution and developing targeted therapies.
- CRISPR-Cas9 technology is fundamental for lineage tracing due to its ability to induce cumulative mutations.
- Homing guide RNA (hgRNA) technology allows for dynamic retargeting and ongoing genetic modifications, enhancing lineage tracing capabilities.
Conclusions:
- The LINEMAP framework significantly advances the capability to reconstruct single-cell lineage using hgRNA technology.
- Findings provide deeper insights into biological mechanisms underlying mutation evolution.
- This work has substantial implications for propelling medical research and developing novel targeted therapies.
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