Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Isolation of Rhizoma Paridis saponins as novel entry inhibitors of Crimean-Congo hemorrhagic fever virus.

Cell insight·2026

Same author

Differential inhibition of Morbillivirus and Henipavirus polymerases by ERDRP-0519 and structure-guided inhibitor optimization.

Cell·2026

Same author

Intrinsically Conductive π-d Conjugated Layers with Co-N<sub>4</sub> Active Sites for Efficient Nitrate Electrocatalysis and Zinc-Nitrate Batteries.

ACS applied materials & interfaces·2026

Same author

Selective capture and digital counting of intact HIV pseudovirus using designer DNA nets, tethered motion, and photonic resonator interferometric scattering microscopy.

APL bioengineering·2026

Same author

Discovery of the SARS-CoV-2 Papain-Like Protease Inhibitor MR1-114: From Structure-Based Design to <i>In Vivo</i> Antiviral Efficacy.

Journal of medicinal chemistry·2026

Same author

Challenges and progress toward real-time detection of airborne viral pathogens.

Critical reviews in biotechnology·2026

Search research articles

Related Experiment Video

Updated: Jul 5, 2025

Detection of Viruses from Bioaerosols Using Anion Exchange Resin

Detection of Viruses from Bioaerosols Using Anion Exchange Resin

Published on: August 22, 2018

VLP-Based Model for Study of Airborne Viral Pathogens.

Michael Caffrey¹, Nitin Jayakumar², Veronique Caffrey¹

¹Department of Biochemistry and Molecular Genetics, University of Illinois at Chicago, Chicago, IL 60607.

Biorxiv : the Preprint Server for Biology

|January 23, 2024

Summary

This study introduces a novel virus-like particle (VLP) model system for studying airborne viral pathogens. This non-infectious VLP model enables real-time detection and quantification of aerosolized viral RNA, advancing airborne pathogen research.

More Related Videos

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes

Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes

Published on: September 27, 2014

Related Experiment Videos

Last Updated: Jul 5, 2025

Detection of Viruses from Bioaerosols Using Anion Exchange Resin

Detection of Viruses from Bioaerosols Using Anion Exchange Resin

Published on: August 22, 2018

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes

Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes

Published on: September 27, 2014

Area of Science:

Virology
Aerosol Science
Biotechnology

Background:

The COVID-19 pandemic highlighted the critical need for effective models to study airborne viral pathogens.
Current limitations in studying airborne pathogen distribution hinder the development of surveillance and mitigation strategies.
Existing models often lack the safety or adaptability required for diverse viral research.

Approach:

Developed a novel model system using non-infectious virus-like particles (VLPs) to simulate airborne viral pathogens.
Demonstrated successful aerosolization of VLPs and real-time quantification of their RNA using Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP).
Utilized both fluorescent and colorimetric assays for sensitive detection of aerosolized VLP RNA.

Key Points:

VLPs mimic airborne pathogens in size and surface components, offering a safe alternative to infectious viruses.
The VLP model facilitates the study of pathogens requiring Biosafety Level 3 (BSL3) and BSL4 containment.
This adaptable system allows for the incorporation of various viral envelope proteins and the development of nucleic acid amplification assays.

Conclusions:

The developed VLP model system provides a versatile and safe platform for studying airborne viral pathogens.
This model system enhances our ability to understand airborne pathogen dynamics and develop effective countermeasures.
It supports the development of novel surveillance tools and diagnostic assays for emerging and existing viral threats.