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Related Experiment Video

Updated: Jul 5, 2025

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

Piggybacking functionalized DNA nanostructures into live cell nuclei.

Golbarg M Roozbahani^1,2, Patricia Colosi³, Attila Oravecz^4,5,6,7

¹Department of Physics, The Ohio State University, Columbus, OH, 43210, USA.

Biorxiv : the Preprint Server for Biology

|January 23, 2024

Summary

DNA origami nanodevices can now be delivered into live cell nuclei. Functionalizing nanorods with an antibody against RNA Polymerase II enables nuclear entry and stability for advanced biological research.

Keywords:

DNA nanotechnology DNA origami Nuclear Delivery RNA polymerase II human cells

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Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags

Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags

Published on: January 17, 2019

Area of Science:

Nanotechnology
Molecular Biology
Cell Biology

Background:

DNA origami (DO) are versatile nanodevices with potential applications in vitro and in vivo.
Targeting DO to mammalian cell nuclei is crucial for studying and controlling intracellular biological processes.
Current methods for nuclear delivery of nanostructures are limited.

Conclusions:

Functionalizing DNA origami with antibodies targeting nuclear factors is a highly effective strategy for nanodevice delivery into live cell nuclei.
This approach enables the use of DNA origami for probing, visualizing, and controlling biological processes within the nucleus of live cells.
The demonstrated nuclear delivery method opens new avenues for advanced applications in molecular and cell biology research.