NBD-based colorimetric and ratiometric fluorescent probe in NIR for bisulfite

Area of Science:

• Analytical Chemistry
• Biophysical Chemistry

Background:

• Sulfur dioxide (SO2) derivatives are crucial in biological processes and food chemistry.
• Accurate detection of SO2 derivatives is essential for quality control and biological studies.
• Existing detection methods may lack selectivity or sensitivity.

Purpose of the Study:

• To develop a novel fluorescent probe for sensitive and selective detection of SO2 derivatives.
• To investigate the probe's performance in complex matrices like food and cells.
• To establish a simple and effective detection tool for bisulfite.

Main Methods:

• Design and synthesis of a FRET-ICT based fluorescent probe (NTC) using coumarin-benzothiazole and 4-nitrobenzo[c][1,2,5] oxadiazole (NBD).
• Evaluation of probe NTC's selectivity, response time, and energy transfer efficiency for sulfite/bisulfite detection.
• Application of NTC in test strips for practical detection and in biological samples (cells).

Main Results:

• Probe NTC demonstrated high selectivity and rapid response towards sulfite (SO32-) and bisulfite (HSO3-).
• Achieved a high Förster Resonance Energy Transfer-Internal Charge Transfer (FRET-ICT) efficiency of 94%.
• Successfully applied NTC in test strips for easy bisulfite detection and in cellular imaging.

Conclusions:

• NTC is a highly efficient fluorescent probe for detecting SO2 derivatives in the NIR region.
• The developed probe and test strips offer a simple, effective, and sensitive tool for bisulfite detection.
• NTC shows potential for real-world applications in food analysis and biological research.