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Published on: January 13, 2023
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A Comparison of Two Macroinvertebrate Multi-Plate Sampling Methods to Inform Great Lakes Monitoring and Remediation
Roger Yeardley1, Brian Duffy2, Kimani Kimbrough3
1Office of Research and Development, United States Environmental Protection Agency, Cincinnati, USA.
Summary
Two Hester-Dendy (HD) sampler versions used for macroinvertebrate bioassessment showed comparable water quality results, despite minor differences in community data influenced by deployment position.
Area of Science:
- Environmental Science
- Ecology
- Water Quality Assessment
Background:
- Hester-Dendy (HD) samplers are standard for macroinvertebrate bioassessment by government agencies.
- The Niagara River, a Great Lakes Area of Concern, required a study to identify tributary impacts on water quality.
Purpose of the Study:
- To compare the effectiveness of two distinct HD sampler versions in assessing macroinvertebrate communities in urban tributaries.
- To evaluate the impact of different sampling methods on water quality bioassessment outcomes.
Main Methods:
- Two co-located HD samplers (NYSDEC and US EPA) were deployed in urban tributaries of the Niagara River.
- Macroinvertebrate communities were analyzed using multivariate ordination (NMDS, MRBP) and various ecological indices.
- Sampler variations included plate number/size, spacing, and deployment method.
Main Results:
- Both HD sampler methods detected community differences across stations, though some grouping by month and method occurred.
- Water quality assessments from the two methods were comparable but not identical.
- No statistical difference was found in total taxa or EPT richness between samplers.
- Significant differences were observed in metrics like percent tolerant collector-gatherer individuals.
Conclusions:
- Despite methodological variations, both HD sampler versions provide robust and comparable water quality assessments.
- Sampler deployment position was identified as a primary factor influencing observed community differences.
- The study validates the use of HD samplers for bioassessment in complex urban watersheds.

