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The dysfunction in intestinal microorganisms and enzyme activity as significant contributors to diarrhea with
Mengsi Zhou1, Xiaoya Li2, Xuehong Wang3
1College of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan, China.
Object:
To investigate the pathogenesis of diarrhea with kidney-yang deficiency syndrome by examining characteristic changes in intestinal microorganisms, enzyme activities, oxidative stress, and metabolism indices.
Methods:
Twenty mice were randomly and equally divided into control group (NC) and model group (NM). Mice in NM group received adenine suspension at a dosage of 50 mg/(kg⋅day) by gavage, 0.4 mL/time, once a day for 14 days, and Folium sennae decoction at a dosage of 10 g/(kg⋅day) by gavage, 0.4 mL/time, once a day for 7 days, starting on 8th day. Mice in NC group were administered an equivalent amount of sterile water by gavage once a day for 7 days, and twice a day from the 8th day. After modeling, assessments encompassed microbial culture, organ index calculation, microbial and enzyme activity detection, malondialdehyde (MDA) content determination, superoxide dismutase (SOD) activity, blood biochemical tests, and observation of kidney tissue pathological changes.
Results:
The results showed that in NM group, a reduction in the number of Lactobacillus and Bifidobacteria was noted, accompanied by an increase in the number of bacteria and E. coli. Xylanase activity in the intestinal contents and mucosa, protease activity in the intestinal mucosa, and intestinal mucosa microbial activity were diminished. Conversely, the activities of amylase, sucrase, and lactase increased in intestinal mucosa. Additionally, there was an elevation in the level of MDA. Renal tubular dilatation and inflammatory cell infiltration were observed in the renal interstitium.
Conclusion:
These dysfunctions in intestinal microorganisms and enzyme activities suggest potential involvement in diarrhea with kidney-yang deficiency syndrome.
Insights
Diarrhea with kidney-yang deficiency syndrome alters gut microbiota, decreasing beneficial bacteria like Lactobacillus and Bifidobacteria. This study reveals associated changes in intestinal enzyme activity and oxidative stress.
Area of Science:
- Traditional Chinese Medicine
- Gastroenterology
- Microbiology
Background:
- Diarrhea associated with kidney-yang deficiency syndrome is a complex condition.
- Understanding its pathogenesis requires examining the interplay between the gut microbiome, enzyme activity, and metabolic markers.
Purpose of the Study:
- To investigate the pathogenesis of diarrhea with kidney-yang deficiency syndrome.
- To analyze characteristic changes in intestinal microorganisms, enzyme activities, oxidative stress, and metabolism indices in a mouse model.
Main Methods:
- A mouse model of diarrhea with kidney-yang deficiency syndrome was established using adenine and Folium sennae decoction.
- Assessments included microbial culture, enzyme activity assays, malondialdehyde (MDA) and superoxide dismutase (SOD) measurements, and histopathological examination of kidney tissues.
Main Results:
- The model group showed reduced Lactobacillus and Bifidobacteria, increased overall bacteria and E. coli, and altered intestinal enzyme activities (decreased xylanase, protease; increased amylase, sucrase, lactase).
- Elevated MDA levels and kidney pathology (tubular dilatation, inflammation) were observed in the model group.
- Intestinal mucosa microbial activity was diminished.
Conclusions:
- Dysbiosis in intestinal microorganisms and altered enzyme activities are implicated in the pathogenesis of diarrhea with kidney-yang deficiency syndrome.
- These findings highlight the role of gut health in this specific syndrome.
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